Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: p53 status of each GBM cell line.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Mutagenesis

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Diverse expression of cell death-related genes by TTFields in GBM cell lines (U87, U251, U373, and T98G) following TTFields treatment. ( a ) Venn diagram of cell death-related genes by TTFields in four indicated GBM cell lines. The groups were divided by TP53 status into the WT (U87) and MT (U251, U373, T98G) TP53 groups. ( b ) Expression of common genes in cell death after TTFields treatment represented by clustering analysis in WT and MT TP53 cells. ( c ) Overlapping MT TP53 cells (U251, U373, and T98G). ( d ) Heat map showing the effects of altered genes by TTFields on cell death in MT TP53 cells. Increased and decreased gene expression is indicated in red and blue colours, respectively. All data represent > 1.5-fold changed genes.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Expressing, Gene Expression

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Differential expression of cell cycle-related genes by TTFields in GBM cell lines. ( a ) Venn diagram of cell cycle-related genes by TTFields in GBM cell lines. ( b ) Expression of common genes related to cell cycle by TTFields in GBM cells. ( c ) Overlapping MT TP53 cells (U251, U373, and T98G). ( d ) Heat map showing cell cycle-related genes with > 1.5-fold change in expression following TTFields treatment in MT TP53 cells. Each spot indicates altered gene after TTFields treatment in the cells. Intensity is represented in red (increased) and blue (decreased) colours. All data represent ≥ 1.5-fold change in gene expression.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Quantitative Proteomics, Expressing, Gene Expression

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Gene expression profiling of GBM cell lines by TTFields on immune and inflammatory responses. ( a ) Venn diagram of immune and inflammatory response-related genes altered by TTFields treatment in GBM cell lines. ( b ) Expression of common genes involved in immune and inflammatory responses in GBM cells following TTFields treatment, as indicated by clustering analysis. ( c ) Overlapping results in MT TP53 cells (U251, U373, and T98G). ( d ) Heat map presents immune and inflammatory response-related genes showing ≥ 1.5-fold change in expression following TTFields treatment in MT TP53 cells. Intensity is indicated by red (increased) and blue (decreased). All data represent ≥ 1.5-fold change in gene expression.

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Gene Expression, Expressing

Journal: Scientific Reports

Article Title: Gene expression profiling of glioblastoma cell lines depending on TP53 status after tumor-treating fields (TTFields) treatment

doi: 10.1038/s41598-020-68473-6

Figure Lengend Snippet: Validation of gene expression in microarray data by qRT-PCR. ( a ) Co-expression of mRNAs of identified genes and TP53 in patients with GBM. ( b – d ) qRT-PCR results for GPNMB, KRT19, and KRT15 expressed as log2 gene expression changes (ΔΔCt). qRT-PCR data are presented as the mean ± standard deviation (n = 3).

Article Snippet: TP53 status and short tandem repeat (STR) analysis of four GBM cell lines were examined by Macrogen, Inc. (Seoul, Korea) and Cosmogenetech, Ltd. (Seoul, Korea) (data not shown).

Techniques: Biomarker Discovery, Gene Expression, Microarray, Quantitative RT-PCR, Expressing, Standard Deviation